This whitepaper is a complete guide to inmunohistochemistry, exploring in great detail the state of the art in this field. This guide provides a starting point for those researchers who are taking their first steps on inmunohistochemistry, and at the same time acts as a chance for the more veteran ones to keep up to date with the latest developments on the field.
In the guide you Will find: experimental desing, sample preparation and fixation, antigen retrieval, blocking and detection, microscopy, quantitative image processing, controls and troubleshooting.
(IHC) and immunocytochemistry (ICC) are powerful techniques that use the antobody-antigen affinity to detect antigens (proteins). Antibody-based applications rely on the specific binding of antibody to the target epitope to generate accurate expression data. Antibodies specifically recognize the epitope of interest through intermolecular forces including hydrophobic interactions, ionic interactions, hydrogen bonding, and others.